QUESTIONS ABOUT FLUOROMETER

1. What device is used to measure the intensity of the fluorescence?

2. True or False. If a substance is in solution, the light's absorbed intensity is usually proportional to the amount of the unknown solution.

3. What is the relationship between the wavelength and the intensity of the emitted radiation?

4. What is the relationship between the wavelength of the radiation and the maximum intensity of the emitted radiation by an activated substance?

5-7. Enumerate at least 3 types of radiation sources used in spectrofluorometry.

8. Which among the radiation sources is often used in spectrofluorometry?

9. Type of fluorometer which utilizes monochromators to separate the fluorescent light from incident light.

10. Type of fluorometer which uses filters to separate the fluorescent light from incident light.

Fluorometric Method

Flourometers are instruments that measures the intensity of the fluorescence. The molecules of certain materials can absorb light energy of certain wavelenghts and re-emit that energy as light of a different wavelength. The wavelengths of the light absored and of the light emitted are characteristic for the substance involved. If this substance is in solution, the intensity of the light emitted is usually proportional to the intensity of light absorbed and to the concentration of the substance in solution.

Reference:
http://apps.who.int/phint/en/p/docf/
http://en.wikipedia.org/wiki/Fluorescence_spectroscopy
Photo: by B Lindy